Quantitating T cell cross-reactivity for unrelated peptide antigens.

نویسندگان

  • Jeffrey Ishizuka
  • Kristie Grebe
  • Eugene Shenderov
  • Bjoern Peters
  • Qiongyu Chen
  • Yanchun Peng
  • Lili Wang
  • Tao Dong
  • Valerie Pasquetto
  • Carla Oseroff
  • John Sidney
  • Heather Hickman
  • Vincenzo Cerundolo
  • Alessandro Sette
  • Jack R Bennink
  • Andrew McMichael
  • Jonathan W Yewdell
چکیده

Quantitating the frequency of T cell cross-reactivity to unrelated peptides is essential to understanding T cell responses in infectious and autoimmune diseases. Here we used 15 mouse or human CD8+ T cell clones (11 antiviral, 4 anti-self) in conjunction with a large library of defined synthetic peptides to examine nearly 30,000 TCR-peptide MHC class I interactions for cross-reactions. We identified a single cross-reaction consisting of an anti-self TCR recognizing a poxvirus peptide at relatively low sensitivity. We failed to identify any cross-reactions between the synthetic peptides in the panel and polyclonal CD8+ T cells raised to viral or alloantigens. These findings provide the best estimate to date of the frequency of T cell cross-reactivity to unrelated peptides ( approximately 1/30,000), explaining why cross-reactions between unrelated pathogens are infrequently encountered and providing a critical parameter for understanding the scope of self-tolerance.

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عنوان ژورنال:
  • Journal of immunology

دوره 183 7  شماره 

صفحات  -

تاریخ انتشار 2009